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Figure 6 | BMC Plant Biology

Figure 6

From: Triacylglycerol synthesis by PDAT1 in the absence of DGAT1 activity is dependent on re-acylation of LPC by LPCAT2

Figure 6

Relative LPCAT activity in protein fractions from mid-developing siliques containing WT and AS11 embryos at stages 7-8 (as designated in Methods"). LPCAT activity was monitored by appearance of (A) 3 H and (B) 14 C label in PC, during a 20 min incubation of WT or AS11 protein in the presence of L-α-palmitoyl- [1-3 H methyl] lyso-3-phosphatidylcholine and [1-14 C] 18:1-CoA. The data represent the average of two experimental replicates ± S.D. While absolute values differed, similar trends were observed in a second independent biological replication.

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