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Table 2 Mutations identified in bread wheat SBEIIa

From: Development of high amylose wheat through TILLING

Gene

DNA

Protein

PSSM

SIFT

SBEIIaA

G5267A

W436*

  
 

G5268A

D437N

7.9

0.04

 

G5289A

G444R

19.0

0.00

 

G5298A

E447K

8.9

0.02

 

G5301A

SJ

  
 

G5418A

R457K

18.3

0.01

 

G5422A

W458*

  
 

G5432A

E462K

17.6

0.01

 

G5448A

G467E

27.1

0.00

 

G5465A

V473M

17.1

0.00

 

C5484T

T479I

10.3

0.40

 

C5712T

T488I

16.9

0.00

SBEIIaB

C4998T

H423Y

15.5

0.59

 

G5036A

M435I

15.0

0.03

 

G5039A

W436*

  
 

G5040A

D437N

19.9

0.01

 

C5044T

S438F

12.1

0.01

 

G5068A

W446*

  
 

G5069A

W446*

  
 

G5161A

V448I

 

0.01

 

G5168A

R450K

19.0

0.01

 

G5185A

A456T

13.3

0.11

 

G5193A

W458*

  
 

G5200A

E461K

18.3

0.01

 

G5203A

E462K

18.3

0.00

 

G5219A

G467E

27.7

0.00

 

C5224T

R469*

  
 

G5234A

G472E

27.7

0.00

 

G5272A

SJ

  
 

G5472A

SJ

  
 

G5475A

M485I

 

0.18

 

C5575T

P519S

17.4

0.02

SBEIIaD

G5202A

W432*

  
 

G5225A

G440E

17.3

0.00

 

G5232A

W442*

  
 

C5423T

H477Y

21.5

0.00

  1. DNA and protein refer to the nucleotide and amino acid changes resulting from the mutation. SJ refers to a splice junction mutation and the symbol * indicates a stop mutation. Mutation severity is predicted using the PARSESNP and SIFT programs [36, 37]. Mutations are predicted to have a severe effect on protein function if PSSM scores are >10 and and SIFT scores are <0.05.