Figure 8
Effects of nigericin, DTT and tentoxin on the response of Chl- a fluorescence to illumination with far red light in dark-adapted wild-type and double mutant plants. The time course experiments were conducted with a PAM 2100 fluorometer and show the response of Chl-a fluorescence yields of Col-0 wild-type or adg1-1/tpt-2 double mutant plants toward far red (FR) illumination in the absence (control, A) or presence of nigericin (B), DTT (C) or tentoxin (D). Prior to the measurements detached leaves were incubated either in 0.05% ethanol (control) or inhibitor solutions for 1 h in the dark. During the course of the experiment a saturation light pulse (SP) was applied after 5 s in order to determine Fo and Fm. Where indicated by arrows, FR was either switched on (+) or off (-). SP indicates the application of saturation light pulses. (E) Impact of nigericin (red circles), DTT (blue circles) or tentoxin (purple circles) on the decay of maximum Chl-a fluorescence yield (Fm') in leaves of Col-0 and adg1-1/tpt-2 plants during illumination with AL at a PDF of 600 μmol·m -2·s -1 compared to the control (black circles). The data for adg1-1/tpt-2 represent the mean ± SE of n = 3 independent experiment