Figure 8
Development of pollen tubes. Pollen tube growth was followed in cross-pollinated pistils: WT/triple – wild type (WT) pistil was pollinated with xi-1/xi-2/xi-k; triple/WT – xi-1/xi-2/xi-k pistil was pollinated with WT. Note the number of pollen grains attached to the stigmas and the number of pollen tubes formed. In the case of triple/WT, WT pollen grains were not able to attach effectively on the surface of xi-1/xi-2/xi-k stigma and only few pollen tubes were formed. Aniline blue staining of pollen tubes was performed 12 hours after hand-pollination. Pg – pollen grains, St – stigma, Pt – pollen tubes. Bar = 50 μm.