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Table 1 Summary of RT-PCR reactions performed for selected TDFs a

From: Genome-wide identification of differentially expressed genes under water deficit stress in upland cotton (Gossypium hirsutum L.)

TDF ID

Description

Category

EST

cDNA-AFLPb

Sizì (bp)

RT-PCRd

02 C11

Hypothetical

Unclassified

DT468917

up R

161

N

02 H11

Hypothetical

DW510601

up R

208

Y

02A12

Hypothetical

DW498397

up R

244

Y

06E08

no homology

na*

up R

186

Y

06B01

glycosyl transferase family 1 protein

ES813597

up L

225

N

01D09

nucleic acid binding protein/cold shock induced

Stress/Defense

ES820443

up R

268

Y

01B11

chloroplast heat shock protein 70.1

DT572113

up L up R

176

Y

02D03

glutathione S-transferase

DW495591

up L

320

N

02A05

Lea5-D/Drought induced

DW228770

up R

233

Y

02A09

manganese superoxide dismutase-like protein

DW226492

dn R

209

N

03E11

universal stress protein

DW484973

up R

350

N

04B09

Hsp70

DW486235

up R

275

Y

02 C06

phosphoethanolamine N-methyltransferase

DW238205

up R

505

N

07B12

phosphoadenosine phosphosulfate reductase family protein

EY197850

up R

273

Y

A04H12

Cu/Zn SOD

JG454758

up R

338

Y

03A07

beta-ketoacyl-ACP synthase II

Metabolism

DW238354

up R

333

Y

08 H05

ribose-5-phosphate isomerase

DT556372

up L

227

Y

08D03

mannose-6-phosphate isomerase

DW497531

up R

175

N

03 H01

MADS-box protein (AGL84)

Gene Regulation

ES839612

up L

151

N

02 F09

translation initiation factor-like protein

EX172706

up R

230

Y

04 G11

RAN2; GTP binding

Signal Transduction

ES827152

dn R

180

Y

08 H03

Lipoxigenase 3

DT567650

up R

190

Y

03 F02

oligo Peptide transporter, putative

Transport

DW502711

dn R

200

N

06 C03

aquaporin PIP1;3/1;4

DQ402075

up R

466

Y

06E09

aquaporin PIP1;12

GU998829

up L

313

N

   

dn R

  

08A01

NITRATE TRANSPORTER1/PEPTIDE TRANSPORTER (POT/NAXT) family

na

dn R

300

Y

04D09

Serine carboxypeptidase

Protein Fate

DT456670

dn R

200

Y

01E10

Photosystem I reaction center subunit VI

Photosynthesis

EY197220

up L

189

N

07E12

Cytochrome b6-f complex

 

ES845215

dn L

285

N

07E02

hydrolase

Cell Growth/Structure

DT465622

dn L

189

N

A5B01

Expansin

 

DW494258

dn L

278

Y

  1. aAll reactions were replicated at least twice and two separate gel electrophoresis were performed per reaction.
  2. bup and dn represent up-regulation and down-regulation, respectively in root (R) and leaf (L) tissues.
  3. cexpected size from PCR amplification.
  4. dRT-PCR results which correspond to cDNA-AFLP data (Y) and do not (N).
  5. *Not available.