Figure 1

Phylogenetic and subcellular predictions of plant NAD(P)H dehydrogenases. Phylogenetic tree of NAD(P)H dehydrogenases from Vovox carteri (Vc), Chlamydomonas reinhardtii (Cr), Physcomitrella patens (Pp), Selaginella moellendorffii (Sm), Oryza sativa (Os), Zea mays (Zm), Vitis vinifera (Vv), Arabidopsis thaliana (At), Glycine Max (Gm), Picea glauca (Pg) and Populus trichocarpa (Pt). The sequences of the Saccharomyces cerevisiae (Sc) NAD(P)H dehydrogenases are included as well as NADH dehydrogenases from Synechocystis sp. PCC 6803 (Synechocystis) and Escherichia coli (E. coli). The predicted and experimental localizations of each protein (including this study) are indicated by the coloured boxes. Orange = Mitochondrial, Green = Plastid, Blue = Peroxisomal and Black = no prediction. Shown also are the last 12 amino acids (chosen because the PTS1 predictor uses the last 12 amino acids), putative peroxisomal targeting signals (PTS1) are coloured depending on the strength as determined by the AraPerox database (dark blue - major PTS1, light blue - minor PTS1 and pink - rare PTS1). The known experimental loactions for Arabidopsis thaliana ND proteins were taken from [23, 25], for Chlamydomonas reinhardtii NDA1 and NDA2 from [32–34] and for Saccharomyces cerevisiae Ndi1, Nde1 and Nde2 from [35]. The phylogenetic tree was constructed using the neighboring-joining method. Numbers at each node are the percentage bootstrap values of 1000 replicates. The scale bar indicates the number of amino acid substitutions at each site.