Figure 3
Pathogen-Induced PR Gene Expression in Atelp3-10 npr1 and Atelp3-10 Plants. (A) Expression of PR genes in Psm ES4326-infected WT, npr1, Atelp3-10 npr1, Com(n), Atelp3-10, and Com(C) plants. Com(n), 35S::AtELP3 Atelp3-10 npr1 transgenic plants; Com(C), 35S::AtELP3 Atelp3-10 transgenic plants. HPI, hours postinoculation. Plants were inoculated with Psm ES4326 (OD600 = 0.001). Leaf tissues were collected at the indicated time points after the inoculation. Total RNA was extracted and subjected to RNA gel blot analysis. The RNA samples were run on the same agarose gel and transferred onto the same blotting membrane. The rRNA bands in the ethidium bromide-stained gel were photographed as a loading control prior to blotting. (B) Growth of Psm ES4326 in WT, Atelp3-10, and Com(C) plants. Cfu, colony-forming units. Leaves of four-week-old plants were inoculated with Psm ES4326 (OD600 = 0.0001). The in planta bacterial titers were determined immediately and three days postinoculation. Data represent the mean of eight independent samples with SD. Different letters above the bars indicate significant differences (p < 0.05, Student’s t-test).