Figure 4
SA-Induced PR Gene Expression and Resistance in Atelp3-10. (A) Expression of PR genes in SA-treated WT, Atelp3-10, and Com(C) plants. Com(C), 35S::AtELP3 Atelp3-10 transgenic plants. Plants were treated with soil drenches of 1 mM SA water solution or water. Leaf tissue was collected at the indicated time points after the treatment. Total RNA was extracted and subjected to RNA gel blot analysis. The rRNA bands in the ethidium bromide-stained gel were photographed as a loading control prior to blotting. (B) Growth of Psm ES4326 in SA-treated WT, Atelp3-10, and Com(C) plants. Plants were treated with soil drenches of 1 mM SA solution or water. Twenty-four hours later, the plants were inoculated with Psm ES4326 (OD600 = 0.001). The in planta bacterial titers were determined immediately and three days postinoculation. Cfu, colony-forming units. Data represent the mean of eight independent samples with SD. Different letters above the bars indicate significant differences (p < 0.05, Student’s t-test).