Figure 4
Expression of ICS1, PDF1.2 and PAP5 in wild-type and pap5-1 plants after Pst DC3000 infection. Transcript levels of ICS1, PDF1.2 and PAP5 in wild-type and pap5-1 plants were quantified after spray inoculation with virulent Pst DC3000 (108 c.f.u ml –l). A, Expression ICS1 and PDF1.2 following Pst DC3000 infection. Total RNA was extracted from leaf tissues sampled at 24 h.p.i. Transcript levels were normalized to the expression of GAPDH in the same samples. The transcript levels were expressed relative to mock infected wild-type plants. B, Expression of PAP5 following Pst DC3000 infection. Total RNA was extracted from leaf tissues 6 h.p.i. Transcript levels were normalized to the expression of GAPDH in the same samples and expressed relative to transcript levels of infected wild-type plants. The bars represent the mean and standard deviation from two independent experiments. Significant differences (P<0.05) are indicated by different letters. Asterisks indicate significant difference in transcript levels compared to wild-type (Students t-test; P<0.05).