Figure 3

Subcellular localization of YFP-tagged AtCuAO1, AtCuAO2 and AtCuAO3 proteins. Confocal microscopic images showing the fluorescence localization in agroinfiltrated N. benthamiana epidermal leaves transiently co-expressing AtCuAO1-YFP (A) with the extracellular protein marker Spg-DsRed (B) or AtCuAO2-YFP (E) or YFP-AtCuAO3 (I) with the peroxisome protein marker CFP-SKL (F, J). Co-localization evaluation of YFP-tagged AtCuAO proteins with the extracellular (C and D) and peroxisome (G, H, K and L) protein markers. Differential interference contrast (DIC) images showing the morphology of transformed cells and merging between the protein of interest, fused to YFP, and the marker (D, H and L). The data presented are from a single representative experiment that was repeated at least three with similar results. Data were obtained using a 40x water immersion objective.