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Table 2 Numerical breakdown of read distribution on the three Illumina lanes for BAT mutation detection in all FAD2 orthologues and BnaC.GL2.b

From: Sequencing-based variant detection in the polyploid crop oilseed rape

Reads

4

5

6

Average

Total

62,071,296

38,839,694

69,785,822

56,898,937

Spike

3,128,126

1,554,316

3,693,148

2,791,863

Unmatched

23,262,622

12,912,128

22,169,416

19,448,055

M13 detected

17,840,274

12,186,625

21,961,629

17,329,509

6 bp BAT

15,859,982

11,258,110

19,398,277

15,505,456

5 bp BAT

591,667

338,953

984,976

638,532

4 bp BAT

304,755

127,279

440,769

290,934

<4 bp BAT

788,808

302,764

891,331

660,968

Munged

32,925,286

23,213,290

40,815,100

32,317,892

Mapped BnaA.FAD2.a

1,566,912

1,420,311

2,143,813

1,710,345

Mapped BnaA.FAD2.b

6,579,001

4,804,826

8,002,197

6,462,008

Mapped BnaC.FAD2.a

791,572

644,265

1,026,912

820,916

Mapped BnaC.GL2.b

12,302,580

6,167,201

15,136,272

11,202,018