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Table 2 Numerical breakdown of read distribution on the three Illumina lanes for BAT mutation detection in all FAD2 orthologues and BnaC.GL2.b

From: Sequencing-based variant detection in the polyploid crop oilseed rape

Reads 4 5 6 Average
Total 62,071,296 38,839,694 69,785,822 56,898,937
Spike 3,128,126 1,554,316 3,693,148 2,791,863
Unmatched 23,262,622 12,912,128 22,169,416 19,448,055
M13 detected 17,840,274 12,186,625 21,961,629 17,329,509
6 bp BAT 15,859,982 11,258,110 19,398,277 15,505,456
5 bp BAT 591,667 338,953 984,976 638,532
4 bp BAT 304,755 127,279 440,769 290,934
<4 bp BAT 788,808 302,764 891,331 660,968
Munged 32,925,286 23,213,290 40,815,100 32,317,892
Mapped BnaA.FAD2.a 1,566,912 1,420,311 2,143,813 1,710,345
Mapped BnaA.FAD2.b 6,579,001 4,804,826 8,002,197 6,462,008
Mapped BnaC.FAD2.a 791,572 644,265 1,026,912 820,916
Mapped BnaC.GL2.b 12,302,580 6,167,201 15,136,272 11,202,018