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Table 3 List of mutations detected within the three Brassica orthologues of FAD2 and BnaC.GL2.b

From: Sequencing-based variant detection in the polyploid crop oilseed rape

Lane BAT Gene Amplicon position Base Reads Ratio Wild type AA Mutant AA
      A C G T    
6 B00146 BnaC.FAD2.a 37 G 183 0 1640 0 0.111585 Glu Lys
5 B00282 BnaC.FAD2.a 45 C 0 3555 0 246 0.069198 Leu Leu
4 B00009 BnaC.FAD2.a 46 C 7 5431 2 77 0.014178 Glu STOP
4 B00282 BnaC.FAD2.a 57 C 0 2032 1 164 0.080709 Ile Ile
4 B00136 BnaC.FAD2.a 59 C 0 2176 0 59 0.027114 Ser Phe
4 B00077 BnaC.FAD2.a 68 G 137 0 1524 1 0.089895 Gly Asp
6 B00087 BnaC.FAD2.a 227 C 0 143 0 14 0.097902 Ser Leu
6 B00040 BnaC.FAD2.a 249 G 11 0 931 0 0.011815 Arg Arg
6 B00207 BnaA.FAD2.a 43 C 3 14174 0 147 0.010371 N/A N/A
4 B00108 BnaA.FAD2.a 45 C 2 12350 0 319 0.02583 N/A N/A
5 B00136 BnaA.FAD2.a 45 C 0 4566 0 298 0.065265 N/A N/A
6 B00041 BnaA.FAD2.a 45 C 1 5883 0 68 0.011559 N/A N/A
4 B00208 BnaA.FAD2.a 48 G 309 0 5574 0 0.055436 N/A N/A
5 B00119 BnaA.FAD2.a 48 G 940 8 15594 0 0.06028 N/A N/A
6 B00114 BnaA.FAD2.a 63 C 1 8285 0 88 0.010622 N/A N/A
6 B00147 BnaA.FAD2.a 63 C 2 9325 0 861 0.092332 N/A N/A
4 B00108 BnaA.FAD2.a 64 G 1065 3 10533 0 0.101111 N/A N/A
6 B00134 BnaA.FAD2.a 69 C 4 8271 0 237 0.028654 N/A N/A
4 B00058 BnaA.FAD2.a 225 C 2 5339 0 316 0.059187 N/A N/A
6 B00075 BnaA.FAD2.a 238 G 181 0 1314 1 0.137747 N/A N/A
4 B00044 BnaA.FAD2.a 248 G 40 0 2640 0 0.015152 N/A N/A
5 B00282 BnaA.FAD2.a 248 G 29 0 846 2 0.034279 N/A N/A
4 B00073 BnaA.FAD2.b 37 G 53 0 1062 0 0.049906 N/A N/A
5 B00285 BnaA.FAD2.b 39 G 27 0 1471 0 0.018355 N/A N/A
5 B00213 BnaA.FAD2.b 39 G 6170 36 59711 4 0.103331 N/A N/A
6 B00128 BnaA.FAD2.b 40 C 9 30818 0 1156 0.037511 N/A N/A
4 B00027 BnaA.FAD2.b 43 C 26 72577 1 6652 0.091654 N/A N/A
4 B00180 BnaA.FAD2.b 45 C 9 26921 0 824 0.030608 N/A N/A
4 B00260 BnaA.FAD2.b 45 C 11 31793 2 1069 0.033624 N/A N/A
5 B00135 BnaA.FAD2.b 45 C 1 4675 0 114 0.024385 N/A N/A
5 B00211 BnaA.FAD2.b 45 C 9 67246 3 5206 0.077417 N/A N/A
5 B00243 BnaA.FAD2.b 45 C 5 87630 1 4416 0.050394 N/A N/A
6 B00299 BnaA.FAD2.b 48 G 335 1 13245 1 0.025293 N/A N/A
4 B00090 BnaA.FAD2.b 59 C 34 127784 1 2404 0.018813 N/A N/A
5 B00087 BnaA.FAD2.b 59 C 3 8544 0 744 0.087079 N/A N/A
5 B00223 BnaA.FAD2.b 63 C 28 64330 0 12219 0.189942 N/A N/A
6 B00250 BnaA.FAD2.b 63 C 2 1965 0 413 0.210178 N/A N/A
5 B00111 BnaA.FAD2.b 68 G 249 1 3179 1 0.078327 N/A N/A
6 B00195 BnaA.FAD2.b 219 C 0 2666 0 28 0.010503 N/A N/A
6 B00118 BnaA.FAD2.b 237 G 20 0 2055 0 0.009732 N/A N/A
5 B00105 BnaA.FAD2.b 265 G 97 0 352 0 0.275568 N/A N/A
6 B00118 BnaC.GL2.b 46 C 14 46512 3 1058 0.022747 intron  
5 B00249 BnaC.GL2.b 65 G 330 2 20270 9 0.01628 Ile Ile
4 B00104 BnaC.GL2.b 68 C 1 7275 1 67 0.00921 Arg Arg
4 B00064 BnaC.GL2.b 109 C 6 32767 4 121 0.003693 Val Met
4 B00307 BnaC.GL2.b 110 C 7 38693 2 784 0.020262 Ala Ala
4 B00076 BnaC.GL2.b 113 C 5 32262 0 70 0.00217 Lys Lys
6 B00091 BnaC.GL2.b 122 C 11 78003 2 1427 0.018294 Leu Leu
6 B00228 BnaC.GL2.b 130 C 5 16523 2 191 0.01156 Ala Thr
5 B00147 BnaC.GL2.b 143 C 1 14670 4 220 0.014997 Trp STOP
6 B00115 BnaC.GL2.b 143 C 17 76370 43 347 0.004544 Trp STOP