From: Sequencing-based variant detection in the polyploid crop oilseed rape
Lane | BAT | Gene | Amplicon position | Base | Reads | Ratio | Wild type AA | Mutant AA | |||
---|---|---|---|---|---|---|---|---|---|---|---|
A | C | G | T | ||||||||
6 | B00146 | BnaC.FAD2.a | 37 | G | 183 | 0 | 1640 | 0 | 0.111585 | Glu | Lys |
5 | B00282 | BnaC.FAD2.a | 45 | C | 0 | 3555 | 0 | 246 | 0.069198 | Leu | Leu |
4 | B00009 | BnaC.FAD2.a | 46 | C | 7 | 5431 | 2 | 77 | 0.014178 | Glu | STOP |
4 | B00282 | BnaC.FAD2.a | 57 | C | 0 | 2032 | 1 | 164 | 0.080709 | Ile | Ile |
4 | B00136 | BnaC.FAD2.a | 59 | C | 0 | 2176 | 0 | 59 | 0.027114 | Ser | Phe |
4 | B00077 | BnaC.FAD2.a | 68 | G | 137 | 0 | 1524 | 1 | 0.089895 | Gly | Asp |
6 | B00087 | BnaC.FAD2.a | 227 | C | 0 | 143 | 0 | 14 | 0.097902 | Ser | Leu |
6 | B00040 | BnaC.FAD2.a | 249 | G | 11 | 0 | 931 | 0 | 0.011815 | Arg | Arg |
6 | B00207 | BnaA.FAD2.a | 43 | C | 3 | 14174 | 0 | 147 | 0.010371 | N/A | N/A |
4 | B00108 | BnaA.FAD2.a | 45 | C | 2 | 12350 | 0 | 319 | 0.02583 | N/A | N/A |
5 | B00136 | BnaA.FAD2.a | 45 | C | 0 | 4566 | 0 | 298 | 0.065265 | N/A | N/A |
6 | B00041 | BnaA.FAD2.a | 45 | C | 1 | 5883 | 0 | 68 | 0.011559 | N/A | N/A |
4 | B00208 | BnaA.FAD2.a | 48 | G | 309 | 0 | 5574 | 0 | 0.055436 | N/A | N/A |
5 | B00119 | BnaA.FAD2.a | 48 | G | 940 | 8 | 15594 | 0 | 0.06028 | N/A | N/A |
6 | B00114 | BnaA.FAD2.a | 63 | C | 1 | 8285 | 0 | 88 | 0.010622 | N/A | N/A |
6 | B00147 | BnaA.FAD2.a | 63 | C | 2 | 9325 | 0 | 861 | 0.092332 | N/A | N/A |
4 | B00108 | BnaA.FAD2.a | 64 | G | 1065 | 3 | 10533 | 0 | 0.101111 | N/A | N/A |
6 | B00134 | BnaA.FAD2.a | 69 | C | 4 | 8271 | 0 | 237 | 0.028654 | N/A | N/A |
4 | B00058 | BnaA.FAD2.a | 225 | C | 2 | 5339 | 0 | 316 | 0.059187 | N/A | N/A |
6 | B00075 | BnaA.FAD2.a | 238 | G | 181 | 0 | 1314 | 1 | 0.137747 | N/A | N/A |
4 | B00044 | BnaA.FAD2.a | 248 | G | 40 | 0 | 2640 | 0 | 0.015152 | N/A | N/A |
5 | B00282 | BnaA.FAD2.a | 248 | G | 29 | 0 | 846 | 2 | 0.034279 | N/A | N/A |
4 | B00073 | BnaA.FAD2.b | 37 | G | 53 | 0 | 1062 | 0 | 0.049906 | N/A | N/A |
5 | B00285 | BnaA.FAD2.b | 39 | G | 27 | 0 | 1471 | 0 | 0.018355 | N/A | N/A |
5 | B00213 | BnaA.FAD2.b | 39 | G | 6170 | 36 | 59711 | 4 | 0.103331 | N/A | N/A |
6 | B00128 | BnaA.FAD2.b | 40 | C | 9 | 30818 | 0 | 1156 | 0.037511 | N/A | N/A |
4 | B00027 | BnaA.FAD2.b | 43 | C | 26 | 72577 | 1 | 6652 | 0.091654 | N/A | N/A |
4 | B00180 | BnaA.FAD2.b | 45 | C | 9 | 26921 | 0 | 824 | 0.030608 | N/A | N/A |
4 | B00260 | BnaA.FAD2.b | 45 | C | 11 | 31793 | 2 | 1069 | 0.033624 | N/A | N/A |
5 | B00135 | BnaA.FAD2.b | 45 | C | 1 | 4675 | 0 | 114 | 0.024385 | N/A | N/A |
5 | B00211 | BnaA.FAD2.b | 45 | C | 9 | 67246 | 3 | 5206 | 0.077417 | N/A | N/A |
5 | B00243 | BnaA.FAD2.b | 45 | C | 5 | 87630 | 1 | 4416 | 0.050394 | N/A | N/A |
6 | B00299 | BnaA.FAD2.b | 48 | G | 335 | 1 | 13245 | 1 | 0.025293 | N/A | N/A |
4 | B00090 | BnaA.FAD2.b | 59 | C | 34 | 127784 | 1 | 2404 | 0.018813 | N/A | N/A |
5 | B00087 | BnaA.FAD2.b | 59 | C | 3 | 8544 | 0 | 744 | 0.087079 | N/A | N/A |
5 | B00223 | BnaA.FAD2.b | 63 | C | 28 | 64330 | 0 | 12219 | 0.189942 | N/A | N/A |
6 | B00250 | BnaA.FAD2.b | 63 | C | 2 | 1965 | 0 | 413 | 0.210178 | N/A | N/A |
5 | B00111 | BnaA.FAD2.b | 68 | G | 249 | 1 | 3179 | 1 | 0.078327 | N/A | N/A |
6 | B00195 | BnaA.FAD2.b | 219 | C | 0 | 2666 | 0 | 28 | 0.010503 | N/A | N/A |
6 | B00118 | BnaA.FAD2.b | 237 | G | 20 | 0 | 2055 | 0 | 0.009732 | N/A | N/A |
5 | B00105 | BnaA.FAD2.b | 265 | G | 97 | 0 | 352 | 0 | 0.275568 | N/A | N/A |
6 | B00118 | BnaC.GL2.b | 46 | C | 14 | 46512 | 3 | 1058 | 0.022747 | intron | |
5 | B00249 | BnaC.GL2.b | 65 | G | 330 | 2 | 20270 | 9 | 0.01628 | Ile | Ile |
4 | B00104 | BnaC.GL2.b | 68 | C | 1 | 7275 | 1 | 67 | 0.00921 | Arg | Arg |
4 | B00064 | BnaC.GL2.b | 109 | C | 6 | 32767 | 4 | 121 | 0.003693 | Val | Met |
4 | B00307 | BnaC.GL2.b | 110 | C | 7 | 38693 | 2 | 784 | 0.020262 | Ala | Ala |
4 | B00076 | BnaC.GL2.b | 113 | C | 5 | 32262 | 0 | 70 | 0.00217 | Lys | Lys |
6 | B00091 | BnaC.GL2.b | 122 | C | 11 | 78003 | 2 | 1427 | 0.018294 | Leu | Leu |
6 | B00228 | BnaC.GL2.b | 130 | C | 5 | 16523 | 2 | 191 | 0.01156 | Ala | Thr |
5 | B00147 | BnaC.GL2.b | 143 | C | 1 | 14670 | 4 | 220 | 0.014997 | Trp | STOP |
6 | B00115 | BnaC.GL2.b | 143 | C | 17 | 76370 | 43 | 347 | 0.004544 | Trp | STOP |