Figure 5From: Identification and characterization of the zinc-regulated transporters, iron-regulated transporter-like protein (ZIP) gene family in maizeSubcellular localization of ZmZIPs in Arabidopsis mesophyll protoplasts. Full-length coding regions without stop codon of the ZmZIP genes were cloned into the pRTL2GFP vector and the resulting plasmid was transiently transformed into Arabidopsis mesophyll protoplasts by the PEG method. The fluorescence of ER marker is indicated in red, the GFP signal is shown in green and the autofluorescence of the chlorophyll (Chl) is artificially stained in blue. The images were obtained by a confocal microscope, and the cytoplasm localization of GFP is used as a control. The scale bar represents 10 μm.Back to article page