Table 2 Shoot regeneration from cotyledons (C) and hypocotyls (H) of L. campestre cultured on the media containing 1.0 mg L -1 2,4-D (2,4-D1.0) for 1 (1d), 2 (2d), 3 (3d) and 4 (4d) days before being transferred to 0.5 mg L -1 zeatin (z 0.5) for 4 weeks (4w), followed by 2.0 mg L -1 zeatin (z 2.0)*
Treatment | Shoot regeneration %** |
|---|---|
Means ± SD | |
2,4-D 1.0, 1d → z 0.5, 4w→z 2.0, C | 6.5 ± 0.25 b |
2,4-D 1.0, 1d → z 0.5, 4w→z 2.0, H | 0.0 ± 0.00 c |
2,4-D 1.0, 2d → z 0.5, 4w→z 2.0, C | 9.4 ± 0.30 ab |
2,4-D 1.0, 2d → z 0.5, 4w→z 2.0, H | 0.0 ± 0.00 c |
2,4-D 1.0, 3d → z 0.5, 4w→z 2.0, C | 14.0 ± 0.35 a |
2,4-D 1.0, 3d → z 0.5, 4w→z 2.0, H | 0.0 ± 0.00 c |
2,4-D 1.0, 4d → z 0.5, 4w→z 2.0, C | 19.4 ±0.40 a |
2,4-D 1.0, 4d → z 0.5, 4w→z 2.0, H | 0.0 ± 0.00 c |