Table 4 Shoot regeneration from cotyledons (C) and hypocotyls (H) of L. campestre cultured on the medium containing 0.5 mg L -1 2,4-D (2,4-D 0,5) for 1, 2 or 3 days (1d, 2d or 3d), followed by transfer to the media containing either 1.0 mg L -1 zeatin (z1.0) or 1.1 mg L -1 TDZ (TDZ1.1)*
Treatment | Shoot regeneration %** |
|---|---|
Means ± SD | |
2,4-D 0,5, 1d→Z 1.0, C | 3,3 ± 0.18 d |
2,4-D 0,5, 1d→Z 1.0, H | 52.1 ± 0.50 c |
2,4-D 0,5, 2d→Z 1.0, C | 1.6 ± 0.13 d |
2,4-D 0,5, 2d→Z 1.0, H | 65.8 ± 0.48 bc |
2,4-D 0,5, 1d→TDZ 1.1, C | 1.7 ± 0.13 d |
2,4-D 0,5, 1d→TDZ 1.1, H | 88.0 ± 0.33 a |
2,4-D 0,5, 2d→TDZ 1.1, C | 3.2 ± 0.18 d |
2,4-D 0,5, 2d→TDZ 1.1, H | 83.6 ± 0.37 ab |
2,4-D 0,5, 3d→TDZ 1.1, C | 0.0 ± 0.00 d |
2,4-D 0,5, 3d→TDZ 1.1, H | 92.7 ± 0.26 a |