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Table 6 Shoot regeneration from cotyledons (C) or hypocotyls (H) of L. campestre cultured on the medium containing 1.0 mg L -1 2,4-D (2,4-D1.0) for 3 days (3d), followed by transfer to 0.5 mg L -1 zeatin for 2 weeks (z 0.5, 2w), then to 2.0 mg L -1 zeatin (z 2.0) or 0.44 mg L -1 TDZ for another 2 weeks (TDZ 0.44, 2w), then to 1.1 mg L -1 TDZ*

From: Development of an efficient regeneration and transformation method for the new potential oilseed crop Lepidium campestre

Treatment**

Regeneration %***

 

Means ± SD

2,4-D1.0, 3d →z 0.5, 2w→z 2.0, C, L

10.4 ± 0.30 b

2,4-D1.0, 3d →z 0.5, 2w→z 2.0, H, L

0.0 ± 0.00 c

2,4-D1.0, 3d →z 0.5, 2w→z 2.0, C, D

6.4 ± 0.25 b

2,4-D1.0, 3d →z 0.5, 2w→z 2.0, H, D

0.0 ± 0.00 c

2,4-D1.0, 3d →TDZ 0.44, 2w→TDZ 1.1, C, L

0.0 ± 0.00 c

2,4-D1.0, 3d →TDZ 0.44, 2w→TDZ 1.1, H, L

30.0 ± 0.46 a

2,4-D1.0, 3d →TDZ 0.44, 2w→TDZ 1.1, C, D

0.0 ± 0.00 c

2,4-D1.0, 3d →TDZ 0,44, 2w→TDZ 1.1, H, D

11.1 ± 0.42 b

  1. *The seeds were germinated either in light (L) or dark (D).
  2. **Each treatment contained 30 explants and repeated at least twice and the data are means ±SD. ***Means followed by different letters indicate significant differences at p = 0.05.
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BMC Plant Biology

ISSN: 1471-2229

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