Figure 4From: Interaction between two rice mitogen activated protein kinases and its possible role in plant defenseOsMPK20-4 and OsMPK3 interaction is phosphorylation independent. In vitro phosphorylation assay with bacterially expressed tag free OsMPK3 and His-tagged OsMPK20-4 showed the two proteins do not phosphorylate each other. The activities of the two kinases are shown by using the general MAPK substrate, MBP. The upper panel shows the autoradiograph while the lower panel shows the Coomassie stained protein of the same gel.Back to article page