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Figure 1 | BMC Plant Biology

Figure 1

From: Transcriptomic analysis highlights epigenetic and transcriptional regulation during zygotic embryo development of Pinus pinaster

Figure 1

Microarray hybridization. (A) Staging system (T0 to T7) used for Pinus pinaster zygotic embryo development [15], showing how samples were divided into five developmental groups/time-points representing early embryogenesis (T0 to T2), pre- cotyledonary (T3 and T4), early cotyledonary (T4B), late embryogenesis (T5) and mature embryo (T7). Bar: T0 and T1 = 300 μm; T2, T3 and T4 = 400 μm; T4B = 800 μm; T5, T6 and T5 = 0.1 cm. Three biological replicates of each sample harvested on Day 0 to Day 25 and two technical replicates were used for hybridization with the reference sample, which consisted of a pool containing equal amounts of total RNA from all five time points. (B) Cluster analysis of the thirty replicates generated using MeV with Pearson correlation and average linkage.

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