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Figure 2 | BMC Plant Biology

Figure 2

From: Perception of soft mechanical stress in Arabidopsis leaves activates disease resistance

Figure 2

Induction of a calcium peak and touch-induced genes after SMS. (A) Changes in cytosolic calcium levels after one SMS event at time 0 s were monitored by FRET using the cameleon yellow protein YC3.6. The experiment was repeated 5 times, one representative time-course is presented. A visual illustration of the time course was placed above the curve at the indicated time point. (B) Changes in cytosolic calcium levels after five SMS events at time 0 s were monitored using the calcium sensing protein aequorin. The experiment was repeated 5 times, one representative time-course is presented. (C) The expression of selected touch genes was determined at various times after SMS (10×)(n = 3; ±SD). The experiment was performed three times with similar results. Asterisks indicate statistically significant differences between SMS-treated samples at different time point (0.5,1, 24 h) in comparison to non-treated samples (0), T-Test (p < 0,05).

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