Figure 2

Validation of heat stress regulated transcripts using RT-PCR. Actin was used as the reference gene for normalization. Normalized fold change based on band intensities are indicated under gel picture. PviUT IDs 1. AP13CTG28453; 2. AlamCTG05039; 3. AP13CTG08489; 4. AP13CTG12099; 5. AP13CTG16627; 6. AP13CTG17205; 7. AP13CTG27166; 8. AP13ISTG54654; 9. KanlCTG02147; 10. KanlCTG03814; 11. AP13CTG14872; 12. AP13CTG15627; 13. AP13CTG20898; 14. AP13CTG22556; 15. AP13CTG24007; 16. AP13CTG29766; 17. AP13CTG31850; 18. AP13ISTG42000; 19. AP13ISTG49574; 20. AP13ISTG64132; 21. KanlCTG08905; 22. KanlCTG24811; 23. KanlCTG36075; 24. KanlCTG31732; 25. AP13ISTG61042; 26. AP13ISTG74729; 27. AP13ISTG68627; 28. AP13CTG27495; 29. AP13CTG27835; 30. KanlCTG24141; 31. KanlCTG22090; 32. AP13ISTG70341; 33. AP13CTG29083; 34. KanlSGLT55375; 35. KanlCTG47822; 36. KanlCTG09502; 37. AP13CTG13877; 38. KanlCTG00487; 39. AP13CTG27164; 40. AP13ISTG49055; 41. AP13ISTG32900; 42. AP13ISTG69974 (Sequences for these unigenes can be retrieved from http://switchgrassgenomics.noble.org). The corresponding rice orthologs, gene annotation, primer sequence and PCR cycles are listed in Additional file 1.