Figure 4

The cyp709b3 mutant plant is sensitive to salt stress. A. Rate of dead seedlings at day 5, 6 and 7 after transfer onto NaCl agar plates. Seeds were germinated on MS medium for 4 days and transferred onto MS agar plates supplemented with 0, 100, 150 or 200 mM NaCl. B. Growth of WT and cyp709b3 seedlings on MS medium (left) and medium supplemented with 150 mM NaCl (right) at day 5 after transfer. C. cyp709b1 and cyp709b2 mutant seedlings are not sensitive to salt stress. D. Growth of WT and cyp709b1, cyp709b2-1 and cyp709b3 mutant plants in soil. Seedlings were irrigated with water (left) or 150 mM NaCl (right) after 21 days. These pictures were taken at day 21 after treatment initiation. Error bars indicate SE (n = 3). Statistically different (p value < 0.05) is indicated using asterisks.