Figure 2

Response of hsi2 mutants and 35S:HSI2 overexpressing lines to simulated drought stress. (A) Wilting rates of hsi2 mutants and corresponding wild-types. (B) Leaf relative water content of the hsi2 mutants and corresponding wild-types. (C) Rate of wilting of three independent 35S:HSI2 lines and untransformed wild-type (Col-0). (D) Recovery from drought 24 h upon re-watering. (E) A representative photograph showing the recovery phenotype of Col-0 and 35S:HSI2 line OEx1. For (A) and (B), the hsi2-2 mutant compares with Col-0 and hsi2-5 compares with Col-2; each wild-type and mutant combination were planted in the same sets of containers. Statistical significance, indicated by an asterisk(*), was determined by a paired Student’s t-test (p ≤ 0.05). Data are presented as means of three replicates containing 12 plants each ± standard error of means. In some cases where the standard errors are very small, the graph symbols may obscure the bars. Experiments were repeated at least three times with similar results.