Figure 8
From: The study of a barley epigenetic regulator, HvDME,in seed development and under drought
DNA methylation of HvDME. DNA methylation assays in 10 day drought-treated and untreated Caresse and Demetra seedlings. Upper: A schematic view of the HvDME gene depicted as a bold black line. Pale blue boxes, exons; pink boxes, LTRs. The regions used for McrBC-PCR are underlined and numbered. ATG and TAG codons are indicated. Lower: Analysis of PCR amplification of McrBC-digested and undigested genomic DNA on agarose gels. Genomic DNA was digested with McrBC and PCR amplification followed. (−), no McrBC; (+), digestion with McrBC.