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Table 1 Parameters for conversion of LA, 13-HOD and 13-KOD by glyoxysomes from etiolated cucumber or sunflower seedlings in the optical β-oxidation assay

From: Degradation of lipoxygenase-derived oxylipins by glyoxysomes from sunflower and cucumber cotyledons

 

Cucumber

Sunflower

LA

initial rate (pmol/(s*mg))

1000 – 4500 (5)

400 – 900 (3)

final NADH (μM/μM LA)

4.7 – 5.6 (5)

1.7 – 2.6 (3)

13-HOD

initial rate (pmol/(s*mg))

900 – 3800 (5)

1100 – 2500 (5)

final NADH (μM/μM HOD)

2.7 – 3.6 (5)

2.2 - 3.2 (5)

13-KOD

initial rate (pmol/(s*mg))

624 (1)

780 (1)

final NADH (μM/μM KOD)

0.95 (1)

1.05 (1)

  1. Parameters were determined as described in Figure 1. Final amounts of NADH are determined for substrate concentrations up to 5 μM. The number of individual experiments with independently purified glyoxysomes are given in brackets.