Figure 4From: Identification of a type I Ca2+/Mg2+-dependent endonuclease induced in maize cells exposed to camptothecinSubcellular localization of CaMNUC32 nuclease activity. Maize cell cultures were grown in the absence or presence of 0.5 μM CPT during 20 days, and fractionated into nuclear and cytoplasmic fractions. Equal among of protein from cytoplasmic and nuclear extracts were subjected in parallel to in-gel activity assay and Western-blot analysis. (A) In-gel Ca2+/Mg2+-dependent nuclease activity assay of cytoplasmic and nuclear fractions. Nuclear fraction purity was checked by immunological detection of the nuclear protein Histone-3 (B) and the cytoplasmic enzyme GriP mutase (C). CON, non-treated ells; CPT, cells treated with 0.5 μM CPT; C, cytoplasmic extract; N, nuclear extract.Back to article page