Figure 6

Phylogenetic analysis of type I nucleases. Putative protein sequences of plant species with complete genome sequenced available were obtained from JGI Genome Portal (http://genome.jgi-psf.org/). The phylogenetic tree was inferred by maximum-likelihood method (RaxML) using 100-bootstraps repeats. For each clade, the significant bootstrap nodal support values (higher than 50%) are annotated, while 100% values are represented by black dots. The maize nuclease identified by mass spectrometry (in bold) locates into a specific group composed by only cereal nucleases (white square), clearly different from those represented by ZEN1/AtENDO1 (group 1) and ZEN2/AtENDO2 (group 2), which include other maize members.