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Figure 6 | BMC Plant Biology

Figure 6

From: The M3 phosphorylation motif has been functionally conserved for intracellular trafficking of long-looped PIN-FORMEDs in the Arabidopsis root hair cell

Figure 6

Wortmannin restored M3 phosphorylation-defective PIN2 proteins in root hair cells. (A) Confocal images showing the subcellular localization of ProE7:PIN2, ProE7:3 m1-PIN2, and ProE7:M3-PIN2 in root hair cells. Transgenic seedlings were treated with 20 μM wortmannin (Wort) for 12 h and stained with FM4-64 (2 μM, for 2 h). Representative images are shown. Bar = 10 μm in all cases. (B) Relative intensities of PIN2:GFP signals in root hair cells from 7–9 seedlings. Values are relative to the WT-PIN2 mock treatment. Data represent means ± SE.

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