Figure 9

Leaf number in MYBR1 genotypes. Seeds from WT, mybr1, mybr1xmybr2 and three lines of OxMYBR1 (42–6, 31–3 and 1–7) were germinated on MS plates and 8 d old seedlings were transferred to fresh plates without and with 5 and 10 μM ACC. Leaf number was counted 17 d after transfer. Control and ACC treated experiments were conducted in four replicates and each replicate contained four seedlings per genotype. The experiment was repeated three times. Standard error was calculated at each time point (n = 12). Two-way ANOVA resulted in significant differences between genotypes but no significant difference between control and ACC treatments. There were increased numbers of rosette leaves in OxMYBR1 lines relative to other genotypes (especially line 42–6) and a significantly reduced number of leaves in the mybr1 and mybr2 double mutant relative to most other genotypes with and without ACC. However, ACC treatment qualitatively enhanced differences between genotypes.