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Figure 4 | BMC Plant Biology

Figure 4

From: A high-throughput virus-induced gene silencing protocol identifies genes involved in multi-stress tolerance

Figure 4

High and low temperatures stress tolerance of leaf disks from gene-silenced plants. Three-week-old N. benthamiana plants were inoculated with TRV carrying inserts of a gene of interest and grown in the greenhouse along with TRV::GFP (vector control) and wild-type non-inoculated plants. Leaf disks were collected from these plants at 20 dpi and floated on deionized water and exposed to either high or low temperature stress. A) For high temperature stress, leaf disks were initially incubated at 35°C for 6 h and then at 45°C for 1 h. Another set of disks was maintained under non-stress conditions. Electrical conductivity of the medium was measured at the end of the stress period. B) For low temperature stress, leaf disks were initially incubated at 4°C for 12 h and then at -2°C for 1 h. Another set of samples was maintained under non-stress conditions. Electrical conductivity of the medium was measured at the end of the stress period. Based on electrical conductivity values, cell membrane stability was calculated. Each bar value represents mean ± sd (n = 12) of three independent experiments. Values significantly different from wild-type and vector control plants were indicated by ‘*’, and the rest were non-significant (Student’s t-test, P < 0.05).

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