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First whole genome based microsatellite DNA marker database of tomato for mapping and variety identification

BMC Plant Biology volume 13, Article number: 197 (2013) Cite this article

Abstract

Background

The cultivated tomato is second most consumed vegetable of the world and is an important part of a diverse and balanced diet as a rich source of vitamins, minerals, phenolic antioxidants and antioxidant lycopene having anti-cancer properties. To reap benefit of genomics of the domestic tomato (Solanum lycopersicum L.) unravelled by Tomato Genome Consortium (The Tomato Genome Consortium, 2012), the bulk mining of its markers in totality is imperative and critically required. The solgenomics has limited number of microsatellite DNA markers (2867) pertaining to solanaceae family. As these markers are of linkage map having relative distance, the choice of selected markers based on absolute distance as of physical map is missing. Only limited microsatellite markers with limitations are reported for variety identification thus there is a need for more markers supplementing DUS test and also for traceability of product in global market.

Description

We present here the first whole genome based microsatellite DNA marker database of tomato, TomSatDB (Tomato MicroSatellite Database) with more than 1.4 million markers mined in-silico, using MIcroSAtellite (MISA) tool. To cater the customized needs of wet lab, features with a novelty of an automated primer designing tool is added. TomSatDB (http://cabindb.iasri.res.in/tomsatdb), a user-friendly and freely accessible tool offers chromosome wise as well as location wise search of primers. It is an online relational database based on “three-tier architecture” that catalogues information of microsatellites in MySQL and user-friendly interface developed using PHP (Hypertext Pre Processor).

Conclusion

Besides abiotic stress, tomato is known to have biotic stress due to its susceptibility over 200 diseases caused by pathogenic fungi, bacteria, viruses and nematodes. These markers are expected to pave the way of germplasm management over abiotic and biotic stress as well as improvement through molecular breeding, leading to increased tomato productivity in India as well as other parts of the world. In era of IPR the new variety can be identified based on allelic variation among varieties supplementing DUS test and product traceability.

Background

Tomato (Solanum lycopersicon L.), a new world solanaceous plant is an excellent model for plant genomic research. The genus Solanum is one of the largest angiosperm genera and the genome has 35,000 genes spread over 12 chromosomes and has few high copy number long terminal repeat (LTR) retrotransposons and largely comprised of low-copy DNA [1]. The genome of tomato has been sequenced by The Tomato Genome Consortium in 2012 [1]. A draft sequence of its closest wild relative, i.e. Solanum pimpinellifolium and the potato genome (Solanum tuberosum L.) has also been reported depicting the extent and pattern of similarities and dissimilarities among the three genomes. The tomato genome sequence will have implications on other plant species viz., strawberries, melons, apple etc., which share some characteristics with tomato. Especially common information related to gene and pathway involved in fruit ripening can be potentially applied to other crops also leading to improved fruit quality and reduced cold chain management costs [2].

The leading tomato producing countries of the world are China, USA, India, Turkey, Egypt, and Italy [3]. Globally, USA (23.2%), Germany (16.5%), Russian Federation (9.6%) and United Kingdom (8.3%) are top countries importing tomatoes while Netherlands is the biggest exporter of tomatoes, exporting over 910 346 tons a year and accounting for 20.6% of world export market in tomatoes [4].

Worldwide, tomatoes are an important part of a diverse and balanced diet as a rich source of vitamins, minerals, phenolic antioxidants and anti-oxidant lycopene having anti-cancer properties [5, 6]. A major constraint in tomato production is the loss incurred due to several diseases. Tomato is known to be susceptible to over 200 diseases caused by pathogenic fungi, bacteria, viruses and nematodes [7]. To accelerate conventional plant breeding, Marker Assisted Selection (MAS) with more than 80 resistance genes to major classes of pathogens (fungal, bacterial, virus and nematode) have been used extensively for pyramiding resistance genes [8, 9]. The other essential characteristics for tomato improvement are development of cultivars with broad adaptability, earliness and fruit quality. Over 75,000 accessions of the cultivated and wild species of tomato are maintained in Genebank around the world [10] but relative and absolute differentiation of these accessions need microsatellite (STR) markers. Thus, these bulk whole genome based STR markers are needed for mapping, variety identification and product traceability.

Earlier in silico works for STR mining were not based on whole genome and thus invariably yielded low/very less number of markers, for example just 80 STR were found in entire gene bank search over 2000 sequences. Though the markers were minimum but are highly potential in distinguishing closely related cultivars of tomato [11]. Reported marker density based on in vivo method is relatively less on every chromosome, for example 12th chromosome has just 37 [12]. For plant variety identification along with degree of admixture, STR is always preferred if they are in multiplex mode (for example, Basmati and non-Basmati rice can be differentiated by 8 plex/ single cocktail based PCR) [13]. Such multiplex designing needs much more number of markers to design multiplex with thermodynamic compatibility, which can be accomplished from our large marker dataset. SGN database is having various classes of markers including STR which are chromosome wise and distance wise based on LOD (Logarithm of the Odds) score [14]. Uniformly distributed markers over genome with an average spacing of 10.0 cM [15] and 1.2 cM (ca. 900 KB) [16] are reported but further higher marker density with average spacing of less than 10 KB has not been reported so far.

Our present work aims at development of such first microsatellite marker database based on whole genome based STR mining which is very user-friendly and freely accessible. Also, the feature of user defined primer designing has great advantage in terms of precise selection from each chromosome, from defined location, size of amplicons for ease of rapid genotyping in simple and low cost agarose gel.

Construction and content

Database processing pipeline

The chromosome wise tomato whole genome data available in public domain [17] was downloaded in FASTA format. All the 12 available chromosomes of the genome were chopped into manageable range using PERL script to be put into MIcroSAtellite identification (MISA) tool [18]. The information on STR numbers, motifs, repeat number, length and size of the repeat, repeat type, GC content, start and end position of the repeat and STR sequence were compiled. Around 1.4 million STRs were generated from tomato genome. Scripts in PERL were written to arrange the output of MISA in proper format in order to create the data file to be further imported to MYSQL database.

Option to find STRs from tomato genome specifically based on chromosome location, type of motif, repeat motif and repeat kind are available. The advance option for search is also available for STRs in desired range of GC content, number of base pairs and copy number. Further, selected STRs can be used in wet lab by generating primers with the integrated Primer3 standalone tool [19]. This obviates the need of manual primer designing using tool/ server.

Database architecture

Tomato MicroSatellite Database (TomSatDB) catalogues all the available information of 1.4 million microsatellite repeats of tomato genome taken under study. It is an online relational database with “three-tier architecture” (Figure 1) with a client tier, middle tier and database tier. In first tier, the total in silico STRs mined using MISA is stored in MySQL database. The middle tier flexibility provision according to the user need has been given. Also, the primer3 standalone code has been integrated to compute primers on user request. The third tier of architecture i.e. client end gives the list of multiple primers along with melting temperature, GC content, start position and product size of selected STRs. The use of open-source server-side scripting language i.e. PHP (Hypertext Pre Processor) has been employed to develop this user friendly interface of TomSatDB.

Figure 1
figure 1

Three-tier architecture of TomSatDB.

Full size image

TomSatDB has seven tabs (Home, About, Database, Analysis, Tutorial, Links and Team) where general information of the developed tomato microsatellite database, tomato, microsatellite markers, analysis of the tomato genome have been described. Tutorial contains the guidelines for users and terminologies used in the database contents. TomSatDB houses other useful links related to solanaceae family.

Accessing database

The TomSatDB is very flexible and easy to handle where the user may query for microsatellites over 12 chromosomes, either one or more chromosomes being selected at a time from tomato genome. The searches may further be customized based on various microsatellite characteristics like motif type (mono, di, tri, tetra, penta, hexa), repeat motif and repeat kind (simple and composite).

The user may go for advance search by specifying the location of STRs on chromosome, number of markers in the given range, markers within given range of GC content, number of basepairs and copy numbers. The STRs preferably at equal interval essentially help in identification of QTL and fine mapping of economically important genes based on LOD scores. Figure 2 shows the flow of database search.

Figure 2
figure 2

Flow of search at TomSatDB.

Full size image

Primer3 tool [19] has been integrated in TomSatDB to generate primers of selected STRs. The STRs may be selected with the help of radio button for generation of primers. Provision to design primer for selected STR locus is provided with a template of approximately 1000 base pairs by selecting upto 500 base pairs of both flanking regions. The provided flexibilities would enable researchers to select markers at known location over the desired chromosomes.

Further, each individual STR of a targeted region over chromosome may be used to narrow down location of gene of interest or linked QTL. The users are given flexibility to replace degenerate bases with any of the alternative bases (A,T,G,C) in TomSatDB.

Analysis of tomato genome and relative abundance

The whole genome was analyzed for getting an overview of the tomato genome. It was observed that 87% and 13% of the STR markers were of simple and compound type, respectively. The “mono” repeat type (50.17%) was found to be dominant followed by “di” (26.69%) type (Table 1).The number of “hexa” repeat type (153) was found to be minimum (0.10%). A considerable variation in microsatellite motif length classes in genomes from species to species has been reported [20]. Abundance of di-nucleotide repeats in eukaryotic genome are reported [21, 22] but in our data “mono” repeats are most abundant due to inherent limitation of the NGS technology having more mono nucleotide stretches as sequencing error [23]. The longer the chromosome, proportionately higher is the total repeat content as expected in ubiquitously distributed STR markers [24]. The length of STRs between 9–16 was found to be most occurring (58.08%) followed by 5–8 and >16 as 22.06% and 6.63% respectively of total STR markers. Chromosome 1 is having the highest number of markers while chromosome 6 exhibits minimum number of STR markers. Chromosome 8 shows highest density (177.8 markers/MBp) of markers and chromosome 2 reports minimum density of markers (128.4 markers/MBp), while the relative density of the tomato whole genome is 154.3 markers/MBp, showing that these markers are ubiquitously distributed with homogeneity in terms of distance, which is inherent attribute of microsatellite to be used as marker of choice.

Table 1 Chromosome wise distribution of STRs
Full size table

STR validation

Finding of these in silico mined STR markers needs extensive wet lab validation across all important tomato varieties of the world. An attempt was made for preliminary small in silico validation with available markers of SGN database [14] using PERL script (Table 2). We found extremely low matching of primers (11.71%). The potential reasons for this magnitude in in silico validation could be varietal difference, ESTs derived STRs and potential of null alleles in tomato genome (Heinz variety taken under study). Though some of the primers from crops other than tomato from solanaceae family showed positive validation up to 7.19% which is obviously expected in different species due to null alleles and genomic changes during speciation in heterologous mode use of STR [25].

Table 2 STR validation result of primers from Solgenomics
Full size table

Utility and discussion

Limited attempt of STR development was reported for example, 12th chromosome has just 37 markers by earlier in vivo method, but in silico method has extracted 11245 from very same chromosome giving much more marker density, which is highly desirable [12]. Though the genome size range of solanaceae is varying from 0.950 GB (tomato) to 2.70 GB (capsicum) but this family has fixed 12 chromosomes. Although the gene repertoire and gene order of solanaceae species are well conserved, the cause of the genome-size difference is not known [26].

STR plays important role in mapping, trait improvement, variety development, variety identification and product traceability. Traditionally, characterization of varieties is based on phenotypic observation but it is very difficult to distinguish varieties with very similar morphological characteristics and identification of the cultivars accurately is essential for maintaining cultivar integrity and Plant Breeders’ Rights.

Limited studies have been reported in variety identification of tomato using STR DNA markers. In one study, out of 20 STR markers, only 11 were able to discriminate 47 varieties [27] and in another study, 12 markers could differentiate 34 varieties [28]. Studies based on 6000 SNP markers over 93 varieties have demonstrated that SNP based variety differentiation is also possible [29]. However, in such SNP based studies, the genotyping data of "Moneymaker" and "Moneyberg" varieties were completely identical leading to no differentiation at all. So, more STR markers from tomato genome are warranted to address varietal differentiation and product tractability in the food chain. Also, DNA fingerprinting is an appropriate tool to track and trace the tomato supply chain, ensuring not only authenticity and integrity of the products but also the absence of any possible genetic contamination by other species or unwanted components [30–32].

Such use of STR in plant variety identification is well reported in other crops like barley varieties [33], S. tuberosum ssp. tuberosum[34], sugarcane [35], capsicum [36], eggplant [37] and identification of Basmati rice from that of non-Basmati rice [13]. Also, the microsatellite STR markers are the method of first choice to complement the DUS (Distinctness, Uniformity and Stability) testing procedure [38, 39].

Our STR database can be a useful tool in MAS programme of tomato improvement. Such use of STR in crop improvement is already reported in sorghum [40], tagging stem rust resistance gene Sr35 in wheat [41], Fusarium head blight resistance in wheat [42], leaf rust resistance gene Lr35 in wheat [43] and mapping of resistance gene effective against Karnal bunt pathogen of wheat [44]. Wheat improvement programs to enhance leaf rust resistance using STR markers has been attempted [45]. STR markers are also used for introgression programs for trait improvement, for example Soltol QTLs in rice. The location of the Saltol QTL on chromosome 1 and identification of additional QTLs associated with salt tolerance is well identified [46].

The relative density of the tomato whole genome reported in the study is 154 markers per MBp. This is almost in range with Arabidopsis (157 MBp), the other crops reported are with higher number of markers like cucumber (367 MBp), rice (370–490 MBp), popular (485 MBp), grape (487 MBp), sorghum (818 MBp), soybean (1115 MBp), maize (2365 MBp), wheat (1000 MBp) and pigeon pea (833 MBp). Though a general negative correlation between genome size and STR density in plants has been reported [47] but we found distance between markers are not proportion to size of genome thus small genome size has enough marker density for mapping purpose.

TomSatDB is of great use to tomato breeders in molecular breeding. The customization of this tool for search based on chromosome may be used by breeders for mapping of gene by markers. It is likely to be accessed by biologists engaged in research with diverse objectives in the crop primarily to develop molecular markers and also to understand the functional significance of microsatellites in regulating gene expression and genome evolution. The comprehensive options to search for simple and compound microsatellites repeats in the genic regions allow users to explore new avenues of investigations on these repeats. The primer designing for PCR amplification of desired motifs will facilitate studies on mutability, microsatellite abundance etc. Association of microsatellites with a particular disease or phenotype may also be explored. Microsatellite data can also be used to investigate various anomalies using candidate gene approach. This microsatellite database will serve as an important application for extracting information in order to design experiments in new directions elucidating novel roles and functions of microsatellites. The STR markers (>1.4 m) reported here is not only relevant for tomato germplasm management using MAS against 200 biotic and abiotic stress but also to other crops. This database is expected to be of immense use across globe by respective statutory authorities for variety identification and varietal dispute resolution supplementing DUS test and product traceability.

Conclusions

A total of 146602 STR markers are reported for the first time using whole genome in the database. Though we have reported small attempt of in silico validation in our studies but extensive wet lab validation of these markers is warranted. These markers are expected to pave the way of germplasm management over abiotic and biotic stress as well as improvement through molecular breeding, leading to increased tomato productivity in various parts of the world. The marker reported in our database are ready to use for mapping as well as also for variety identification and product traceability, paving the pathway of best use of genomics and computational tool in endeavor of tomato improvement and variety management at global level.

Availability and requirement

TomSatDB, the tomato microsatellite marker database is freely accessible for research purposes for non-profit and academic organizations at http://cabindb.iasri.res.in/tomsatdb.

References

  1. The Tomato Genome Consortium: The tomato genome sequence provides insights into fleshy fruit evolution. Nature Lett. 2012, 485: 635-641. 10.1038/nature11119.

    Article  Google Scholar 

  2. Ranjan A, Ichihashi Y, Sinha NR: The tomato genome: implications for plant breeding, genomics and evolution. Genome Biol. 2012, 13: 16. 10.1186/gb-2012-13-3-r16.

    Article  Google Scholar 

  3. Food and agriculture organization. http://faostat.fao.org.

  4. Directorate Marketing: A profile of the South African tomato market value chain. Department - Agriculture, forestry and fisheries: Republic of South Africa; 2012.

    Google Scholar 

  5. Rick CM: Tomato. Hybridization of crop plants. Edited by: Fehr WR, Hadley HH. Madison, USA: American Society of Agronomy-Crop Science Society of America;1980, 669-680.

    Google Scholar 

  6. Willcox JK, Catignani GL, Lazarus S: Tomatoes and cardiovascular health. Crit Rev Food Sci Nutri. 2003, 43 (1): 1-18. 10.1080/10408690390826437.

    Article  CAS  Google Scholar 

  7. Lukyanenko AN: Disease resistance in tomato. Genetic Improvement of Tomato. Edited by: Berlin KG. Germany: Springer; 1991, 99-119. Monographs on Theor Appl Genet, vol 14

    Chapter  Google Scholar 

  8. Grube RC, Radwanski E, Jahn M: Comparative genetics of disease resistance within the Solanaceae. Genetics. 2000, 155: 873-88.

    PubMed  CAS  PubMed Central  Google Scholar 

  9. Foolad MR, Panthee DR: Marker-assisted selection in tomato breeding. Crit Rev Plant Sci. 2012, 31 (2): 93-123. 10.1080/07352689.2011.616057.

    Article  Google Scholar 

  10. Larry R, Joanne L: Genetic resources of tomato (Lycopersicon esculentum Mill.) and Wild Relatives. Genetic improvement of solanaceous crops, Volume 2-Tomato. Edited by: Razdan MK, Mattoo AK. New Hampshire, USA: Science Publishers; 2007, 25-75.

    Google Scholar 

  11. Smulders MJM, Bredemeijer G, Rus-Kortekaas W, Arens P, Vosman B: Use of short microsatellites from database sequences to generate polymorphisms among Lycopersicon esculentum cultivars and accessions of other Lycopersicon species. Theor Appl Genet. 1997, 94 (2): 264-272. 10.1007/s001220050409.

    Article  CAS  Google Scholar 

  12. Subramaniam G, Palchamy P, Robert P, Eguru R, Jaw-Fen W: Development of tomato SSR markers from anchored BAC clones of chromosome 12 and their application for genetic diversity analysis and linkage mapping. Euphytica. 2011, 178 (2): 283-295. 10.1007/s10681-010-0331-8.

    Article  Google Scholar 

  13. Archak S, Lakshminarayanareddy V, Nagaraju J: High-throughput multiplex microsatellite marker assay for detection and quantification of adulteration in Basmati rice (Oryza sativa). Electrophoresis. 2007, 28: 2396-2405. 10.1002/elps.200600646.

    Article  PubMed  CAS  Google Scholar 

  14. Sol genomics network. http://solgenomics.net/.

  15. Frary A, Xu Y, Liu J, Mitchell S, Tedeschi E, Tanksley S: Development of a set of PCR-based anchor markers encompassing the tomato genome and evaluation of their usefulness for genetics and breeding experiments. Theor Appl Genet. 2005, 111: 291-312. 10.1007/s00122-005-2023-7.

    Article  PubMed  CAS  Google Scholar 

  16. Tanksley SD, Ganal MW, Prince JP, de Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller J, Miller L, Paterson AH, Pineda O, Riider MS, Wing RA, Wu W, Young ND: High density molecular linkage maps of the tomato and potato genomes. Genetics. 1992, 132: 1141-1160.

    PubMed  CAS  PubMed Central  Google Scholar 

  17. Solanum lycopersicum project. http://mips.helmholtz-muenchen.de/plant/tomato/index.jsp.

  18. MicroSatellite analysis tool. http://pgrc.ipk-gatersleben.de/misa/.

  19. Rozen S, Skaletsky HJ: Primer3 on the WWW for general users and for biologist programmers. Bioinformatics Methods and Protocols: Methods in Molecular Biology. Edited by: Krawetz S, Misener S. 2000, Totowa, NJ: Humana Press, 365-386.

    Google Scholar 

  20. Sharma PC, Grover A, Kahl G: Mining microsatellites in eukaryotic genomes. Trends Biotechnol. 2007, 25 (11): 490-498. 10.1016/j.tibtech.2007.07.013.

    Article  PubMed  CAS  Google Scholar 

  21. Kariin S, Burge C: Dinucleotide relative abundance extremes: a genomic signature. Trends Genet. 1995, 11 (7): 283-290. 10.1016/S0168-9525(00)89076-9.

    Article  Google Scholar 

  22. Shioiri C, Takahata N: Skew of mononucleotide frequencies, relative abundance of dinucleotides and DNA strand asymmetry. J Mol Evol. 2001, 53: 364-376. 10.1007/s002390010226.

    Article  PubMed  CAS  Google Scholar 

  23. Haseneyer G, Schmutzer T, Seidel M: From RNA-seq to large-scale genotyping-genomics resources for rye (Secale cereale L.). BMC Plant Biol. 2011, 11: 131-143. 10.1186/1471-2229-11-131.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  24. Zietkiewicz E, Rafalski A, Labuda D: Genome finger printing by simple sequence repeat (SSR) - anchored polymerase chain reaction amplification. Genomics. 1994, 20: 176-183. 10.1006/geno.1994.1151.

    Article  PubMed  CAS  Google Scholar 

  25. Kim KS, Min MS, An JH, Lee H: Cross-species amplification of bovidae microsatellites and Low diversity of the endangered Korean goral. J Hered. 2004, 95 (6): 521-525. 10.1093/jhered/esh082.

    Article  PubMed  CAS  Google Scholar 

  26. Park M, Jo SH, Kwon J, Park J, Ahn JH, Kim S, Lee YH, Yang TJ, Hur CG, Kang BC, Kim BD, Choi D: Comparative analysis of pepper and tomato reveals euchromatin expansion of pepper genome caused by differential accumulation of Ty3/Gypsy-like elements. BMC Genomics. 2011, 12: 85-97. 10.1186/1471-2164-12-85.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  27. Sardaro MLS, Marmiroli M, Maestri E, Marmiroli N: Genetic characterization of Italian tomato varieties and their traceability in tomato food products. Food Sci Nutri. 2013, 1 (1): 54-62. 10.1002/fsn3.8.

    Article  CAS  Google Scholar 

  28. Srivastava DK, Shoemaker LS, Franks CE, Sussman MD: Single laboratory validation of a microsatellite marker-based method in tomato variety identification. J AOAC Int. 2011, 94 (1): 251-258.

    PubMed  CAS  Google Scholar 

  29. Viquez-Zamora M, Vosman B, van de Geest H, Bovy H, Visser RGF, Finkers R, van Heusden AW: Tomato breeding in the genomics era: insights from a SNP array. BMC Genomics. 2013, 14: 354. 10.1186/1471-2164-14-354.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  30. Marmiroli N, Peano C, Maestri E: Advanced PCR techniques in identifying food components. Food authenticity and traceability. Edited by: Lees M. Cambridge UK: Woodhead Publishing; 2003, 3-33.

    Chapter  Google Scholar 

  31. Marmiroli N, Maestri E, Pafundo S, Vietina M: Molecular traceability of olive oil: From plant genomics to food genomics. Advances in olive resources. Edited by: Berti L, Maury J. Trivandrum, India: Transworld Research Network; 2009, 157-172.

    Google Scholar 

  32. Agrimonti C, Vietina M, Pafundo S, Marmiroli N: The use of food genomics to ensure the traceability of olive oil. Trends Food Sci Tech. 2011, 22: 237-244. 10.1016/j.tifs.2011.02.002.

    Article  CAS  Google Scholar 

  33. Karakousis A, Barr AR, Chalmers KJ, Ablett GA, Holton TA, Henry RJ, Lim P, Langridge P: Potential of SSR markers for plant breeding and variety identification in Australian barley germplasm. Aust J of Agr Res. 2003, 54: 1197-1210. 10.1071/AR02178.

    Article  CAS  Google Scholar 

  34. Kawchuk LM, Martin RF, Mcpherson J: Resistance in transgenic potato expressing the potato leafroll virus coat protein gene. Mol Plant Microbe In. 1990, 3: 301-307. 10.1094/MPMI-3-301.

    Article  CAS  Google Scholar 

  35. Manigbas NL, Villegas LC: Microsatellite Markers in Hybridity tests to identify true hybrids of sugarcane. Philipp J Crop Sci. 2004, 29 (2): 23-32.

    Google Scholar 

  36. Shirasawa K, Ishii K, Kim C, Ban T, Suzuki M, Ito T, Muranaka T, Kobayashi M, Nagata N, Isobe S, Tabata S: Development of Capsicum EST-SSR markers for species identification and in silico mapping onto the tomato genome sequence. Mol Breeding. 2013, 31 (1): 101-110. 10.1007/s11032-012-9774-z.

    Article  CAS  Google Scholar 

  37. Stagel A, Portis E, Toppino L, Rotino GL, Lanteri S: Gene-based microsatellite development for mapping and phylogeny studies in eggplant. BMC Genomics. 2008, 9: 357-370. 10.1186/1471-2164-9-357.

    Article  PubMed  PubMed Central  Google Scholar 

  38. McCouch SR, Chen X, Panaud O, Temnykh S, Xu Y, Cho YG, Huang N, Ishii T, Blair M: Microsatellite marker development, mapping and applications in rice genetics and breeding. Plant Mol Biol. 1997, 35: 89-99. 10.1023/A:1005711431474.

    Article  PubMed  CAS  Google Scholar 

  39. Becher SA, Steinmetz K, Weising K, Boury S, Peltier D, Renou JP, Kahl G, Wolff K: Microsatellites for variety identification in Pelargonium. Theor Appl Genet. 2000, 101: 643-651. 10.1007/s001220051526.

    Article  CAS  Google Scholar 

  40. Wang Y, Bible P, Loganantharaj R, Upadhyaya HD: Identification of SSR markers associated with height using pool-based genome-wide association mapping in sorghum. Mol Breeding. 2012, 30 (1): 281-292. 10.1007/s11032-011-9617-3.

    Article  Google Scholar 

  41. Babiker E, Ibrahim AMH, Yen Y, Stein J: Identification of a microsatellite marker associated with stem rust resistance gene Sr35 in wheat. Aust J Crop Sci. 2009, 3: 195-200.

    CAS  Google Scholar 

  42. Liu S, Anderson JA: Marker assisted evaluation of fusarium head blight resistant wheat germplasm. Crop Sci. 2003, 43: 760-766. 10.2135/cropsci2003.0760.

    Article  CAS  Google Scholar 

  43. Seyfarth R, Feuillet C, Schachermayr G, Winzeler M, Keller B: Development of a molecular marker for the adult plant leaf rust resistance gene Lr35 in wheat. Theor Appl Genet. 1999, 99 (3–4): 554-560.

    Article  PubMed  CAS  Google Scholar 

  44. Singh R, Datta D, Priyamvada , Singh S, Tiwari R: Marker-assisted selection for leaf rust resistance genes Lr19 and Lr24 in wheat (Triticum aestivum L.). Appl Genet. 2004, 45 (4): 399-403.

    Google Scholar 

  45. Kolmer JA, Anderson JA, Flor JM: Chromosome location, linkage with simple sequence repeat markers, and leaf rust resistance conditioned by gene in wheat. Crop Sci. 2010, 50: 2392-2395. 10.2135/cropsci2010.01.0005.

    Article  Google Scholar 

  46. Thomson MJ, de Ocampo M, Egdane J, Rahman MA, Sajise AG, Adorada DL, Tumimbang-Raiz E, Blumwald E, Seraj ZI, Singh RK, Gregorio GB, Ismail AM: Characterizing the Saltol quantitative trait locus for salinity tolerance in rice. Rice. 2010, 3: 148-160. 10.1007/s12284-010-9053-8.

    Article  Google Scholar 

  47. Morgante M, Olivieri AM: PCR-amplified microsatellites as markers in plant genetics. Plant J. 1993, 3: 175-182. 10.1111/j.1365-313X.1993.tb00020.x.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

Financial assistance received by National Agricultural Innovation Project, Indian Council of Agricultural Research, New Delhi entitled “Establishment of National Agriculture Bioinformatics Grid in ICAR” is gratefully acknowledged. The technical assistance of Jai Bhagwan in maintaining the web server and AR Paul in designing the logo of TomSatDB are thankfully acknowledged. Authors acknowledge the critical input of all the anonymous reviewers and editor in improvement of the manuscript.

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  1. Centre for Agricultural Bioinformatics, Indian Agricultural Statistics Research Institute, Library Avenue, New Delhi, 110012, India

    Mir A Iquebal,  Sarika, Vasu Arora, Anil Rai & Dinesh Kumar

  2. Germplasm Exchange Unit, National Bureau of Plant Genetic Resources (NBPGR), New Delhi, 110012, India

    Nidhi Verma

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Authors’ contributions

DK conceived this study. MAI, S and VA created the work-flow, database, web-tool and performed data analyses. MAI, S, NV, DK and AR drafted the manuscript. All authors read and approved the manuscript.

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Iquebal, M.A., Sarika, Arora, V. et al. First whole genome based microsatellite DNA marker database of tomato for mapping and variety identification. BMC Plant Biol 13, 197 (2013). https://0-doi-org.brum.beds.ac.uk/10.1186/1471-2229-13-197

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  • DOI: https://0-doi-org.brum.beds.ac.uk/10.1186/1471-2229-13-197

Keywords

BMC Plant Biology

ISSN: 1471-2229

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