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Figure 3 | BMC Plant Biology

Figure 3

From: TcNPR3 from Theobroma cacao functions as a repressor of the pathogen defense response

Figure 3

Functional complementation of the Arabidopsis npr3-3 mutation by TcNPR3 . Pseudomonas syringae pv. tomato DC3000 infection assay of transgenic npr3-3 mutant overexpressing TcNPR3. A. Representative images of siliques from infected Col-0, npr3-3 mutant and five independent lines of npr3-3 plants transformed with TcNPR3 seven days after inoculation with virulent bacteria Pseudomonas syringae pv. tomato DC3000 (P.s.t.) (OD600 = 0.2 with 0.02% Silwet L-77) on top of the inflorescence. Additionally, water-treated (0.02% Silwet L-77) inflorescences of seven genotypes served as mock inoculation. Size bars are indicated in white (10 mm). B. Average lengths of the fourth silique from the bottom of infected inflorescences seven days after inoculation (gray bars) compared to water mock inoculations (black bars). Bar chart represents means ± SE of siliques from four biological replicates per treatment. Letters above the histogram indicate statistically significant differences among genotypes (P < 0.05) determined by single factor ANOVA. C. Gene expression of TcNPR3 in transgenic Arabidopsis npr3-3 lines. RT-PCR was performed with cDNA prepared from flowers of 6-week-old plants of wild type (C), npr3-3 mutant (n) and five individual transgenic npr3-3 mutant overexpressing TcNPR3 (1–5). Arabidopsis ubiquitin (AtUbiquitin) was assayed as an internal and cDNA loading control.

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