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Table 1 The level of total amino acids in dry wild type (WT) seeds, in seeds expressing F-AtCGS (LF) and in seeds expressing T-AtCGS (LT), after protein hydrolysis

From: Tobacco seeds expressing feedback-insensitive cystathionine gamma-synthase exhibit elevated content of methionine and altered primary metabolic profile

 

WT

LF 29

LF 17

LT 19

LT 30

Alanine

19232 ± 1957

20778 ± 2289

21704 ± 1098

22891 ± 1713

21993 ± 1665

Valine

41269 ± 6044

41332 ± 6002

41269 ± 3322

43454 ± 1418

45885 ± 3045

Serine

18725 ± 1181 B

18090 ± 3097 B

18725 ± 2660 B

28859 ± 130 A

22908 ± 127 A

Leucine

45675 ± 7705

45499 ± 5528

45531 ± 6186

53449 ± 985

55274 ± 1937

Threonine

23410 ± 4732

23187 ± 4212

23410 ± 3357

34249 ± 1026

31440 ± 2247

Isoleucine

30985 ± 4603

31254 ± 1233

31981 ± 1966

32913 ± 599

39781 ± 1098

Proline

11234 ± 1891 B

10954 ± 985 B

11164 ± 1550 B

13196 ± 1439 A

13784 ± 726 A

Glycine

108546 ± 4480

108756 ± 3567

109546 ± 5814

109494 ± 6499

112348 ± 4972

Methionine

4374 ± 315 B

3962 ± 997 B

4199 ± 313 B

6992 ± 459 A

6376 ± 573 A

Aspartate

39625 ± 11058 B

41500 ± 9876 B

40625 ± 7303 B

61360 ± 2182 A

48541 ± 3687 A

Phenylalanine

21500 ± 4772

23058 ± 5562

22003 ± 3568

27960 ± 1025

28166 ± 2235

Glutamate

3058 ± 785 B

2984 ± 689 B

3644 ± 330 B

9217 ± 242 A

5896 ± 564 A

Histidine

2725 ± 666

2769 ± 1072

2863 ± 605

3415 ± 358

3722 ± 231

Lysine

20263 ± 4330

22645 ± 5562

22094 ± 4570

25838 ± 1138

24113 ± 2645

Tyrosine

19841 ± 3273

20476 ± 5797

21195 ± 3165

24230 ± 1115

27420 ± 971

Total

411462 ± 21890 B

417244 ± 18664 B

419953 ± 11284 B

497517 ± 19064 A

487647 ± 13687 A

  1. The levels of amino acids were measured using GC-MS and displayed as nmol/gr dry seeds. Values are representative of two independently grown sets of plants are the mean ± standard deviation of five biological repetitions of 50 mg seeds isolated from three plants per line. Statistically significant changes (p < 0.05, using two-way ANOVA) are identified by letters.