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Table 2 Recognition sites and methylation sensitivities of the restriction enzymes used

From: Vernalization treatment induces site-specific DNA hypermethylation at the VERNALIZATION-A1 (VRN-A1) locus in hexaploid winter wheat

Enzyme

Recognition site

Sensitivity to methylation

Bfa I

none

BstU I

CG

Hpa II

CG

Msp I

CHG

PspG I

CHG/CHH

  1. Arrowheads indicate enzymes cutting sites and bold Cs show methylation sensitive sites.
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BMC Plant Biology

ISSN: 1471-2229

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