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Figure 2 | BMC Plant Biology

Figure 2

From: miR394 and LCR are involved in Arabidopsis salt and drought stress responses in an abscisic acid-dependent manner

Figure 2

Expression of LCR ( at1g27340 ) in developing Arabidopsis and its regulation by abiotic stresses and ABA treatment. A: pLCR::GUS expression pattern in different tissues: GUS expression in germinating seeds (a); cotyledons of four day-old seedlings (b); true leaves of seven day-old seedlings (c); rosette leaves of the thirty day-old plants (d); root tips of fourteen day-old seedlings (e); stems (f); flowering tissues (g); and siliques (h). B: GUS staining in two week-old pLCR::GUS transgenic seedlings treated with salt (300 mM NaCl) (j), drought (k) and 100 μM ABA (l) for 12 h. C/D/E: Real time qRT-PCR and Western-bolt analyses on expression of RNA level of LCR and GUS in pLCR::GUS plants or protein level of LCR in wild-type. Two week-old wild-type Arabidopsis seedlings were treated with NaCl (300 mM), and ABA (100 μM) for 6–12 h. The data were obtained from qRT-PCR (C: pLCR::GUS line-1; D: pLCR::GUS line-2) and Western-bolt analysis (E). Vertical bars represent standard deviation of the mean (n = 3, treatments with 60 seedlings). Asterisks indicate the significant difference between the treatments and control (P<0.05).

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