Figure 4
From: DAYSLEEPER: a nuclear and vesicular-localized protein that is expressed in proliferating tissues
Localization of fluorescent-tagged DAYSLEEPER in protoplasts. Arabidopsis protoplasts transformed with A. 35S::Cerulean:DAYSLEEPER and 35S::SNX1:mRFP1 constructs, B. 35S::Cerulean:DAYSLEEPER and 35S::YFP:SYP61 constructs and C. 35S::TagRFP:DAYSLEEPER and 35S::RHA1:eCFP constructs. From left to right, the panels depict fluorescent-tagged DAYSLEEPER (DAYSLEEPER), a fluorescent-tagged marker gene (marker), a composite image (merge) and a DIC image (DIC). The areas indicated by arrows are shown enlarged. The nucleus is designated with an “N”. The scale bars represent 10 μm.