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Figure 2 | BMC Plant Biology

Figure 2

From: A novel dominant glossy mutation causes suppression of wax biosynthesis pathway and deficiency of cuticular wax in Brassica napus

Figure 2

Epicuticular wax and cuticle layer of WT and the GL mutant. (A-D) Scanning electron microscopy. (A) Wax crystals on the WT leaf were dense, with high proportion of tubular-like wax crystals. (B) Wax crystals sparsely distributed on the leaf of the GL mutant. Bar =20 μm. (C) Wax crystals on the WT stem with a high proportion of plate-like wax crystals. (B) Fewer wax crystals were present on the stem of the GL mutant. Bar =10 μm. (E-H) Transmission electron microscopy. The leaf and stem cuticle proper is indicated by black arrowheads and the cuticle layer is indicated by white arrowheads. The cuticle ultrastructure of GL mutant leaf (F) and stem (H) were altered dramatically compared with that of WT leaf (E) and stem (G). The cuticle membrane (cuticle proper plus cuticular layer) of GL leaf and stems was thicker, and less osmiophilic. Bar =200 nm.

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