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Figure 5 | BMC Plant Biology

Figure 5

From: A novel dominant glossy mutation causes suppression of wax biosynthesis pathway and deficiency of cuticular wax in Brassica napus

Figure 5

Downregulation of cuticular wax biosynthetic genes in the glossy mutant. (A) Venn diagrams showing the overlapping and specific DEGs in the WT vs. mutant and DHNB vs. DHGB analyses. (B) Simplified cuticular wax biosynthetic pathway, as adapted from previous reports [2, 7, 27]. The numbers indicate the mean fold change of the genes belonging to individual gene groups (I to VIII), as marked in (B). The question marks and dotted lines indicate unidentified enzymes and processes, respectively. (C) List of the wax biosynthetic genes and fatty acid synthesis genes down-regulated in both the glossy mutant and glossy DH line 69. The P values were corrected for multiple testing using FDR methodology. The group of genes was classified based on their biochemical function. FC, fold change. (D) qRT-PCR of wax biosynthetic gene expression. Total RNA was extracted from 6-week-old leaves. The transcript levels were examined using qRT-PCR. The bars indicate SE of the mean.

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