Figure 2
The cis-regulatory mechanism and genotype-dependent monoallelic expression. (A) Correlation analysis between genic expression in the parental generation and allelic expression in the F1 generation of the three hybrids. Red, allelic-specific expressed genes (ASE); blue, biallelic expressed genes. (B) The number of variety-specific and commonly expressed monoallelic expressed genes in the three F1 hybrids. (C) Example of a monoallelic expression gene confirmed by RT-PCR sequencing of reciprocal F1 crosses. (D) Confirmation of genotype-dependent monoallelic expression patterns in the three F1 hybrids showing the origin of the alleles. (E) Example of a preferential allelic expression gene confirmed by RT-PCR sequencing of reciprocal F1 crosses. (F) Example of a biallelic expression gene confirmed by RT-PCR sequencing of reciprocal F1 crosses.