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Table 1 Sequences of CLE like peptides and control peptides used in binding assays

From: Homo-dimerization and ligand binding by the leucine-rich repeat domain at RHG1/RFS2 underlying resistance to two soybean pathogens

   

Kd (nM)

Sequence

Name

Synonyms

RHG1

RHG4

RLAPGGPDPQHN

2

GmNICI, LjCLE-R2 and d LjCLE-R1

45

96

DLPLAPADRLAGGPDPQHNVRAPPRKP

2L

GmNICI, LjCLE-R2 and d LjCLE-R1

142

338

RLAPEGPDPHHN

30

GmCLE30, GmRICI

44

84

AHEVPSGPNPISNR

T

GmTDIF, ZeTDIF

20

204

SKRRVPNGPDPIHNR

36

GmCLE34, AtCLE36, MtCLE36

14

52

RAELDFNYMSKRRVPNGPDPIHNRRAGNSGR

36L

GmCLE34, AtCLE36, MtCLE36

49

51

RTVPSGPDPLHH

3

GmCLE3, AtCLE3, AtClv3 unmodified

29

50

KGLGLHEEELRTVPSGPDPLHHHVNPPRQPR

3L

GmCLE3, AtCLE3, AtClv3 unmodified

65

142

KRLSPSGPDPHHH

N

HgCLV3

30

78

CTLSRLKTLDISNNALNGNLPATLSNLS

L

GmLRR, GmRLK18-1

36

135

  1. Consensus sequences within the peptides were underlined. Dashed underline was the LRR peptide fragment used to estimate the Kd of dimerization and to raise the anti-Gm18RLK-1 antibody. Complete annotations can be found in [30]. Binding constants were calculated from titration experiments.