Figure 4

GmPHT1 transcription in the response to the low Pi (1 μM) stress. At least five individual plants per treatment were harvested when the unifoliolate leaves fully expanded. The transcript abundance of GmPHT1 genes in the roots (R), stems (S) and unifoliolate leaves (U) were shown using the expression of the 500 μM Pi treatment group as a control. The geometric means of GmSKIP16 and GmUNKI transcripts were used as the reference transcript. The values are means of three replicates and each replicate represented a pool from at least five plants. Error bars represent SD.