Figure 4From: A qRT-PCR assay for the expression of all Mal d 1 isoallergen genes Ct values of three putative reference genes in ‘Florina’ and ‘Gala’ peel and flesh. The Ct values obtained after qRT-PCR amplification with the primers for actin, UBC and GAPDH in ‘Florina’ and ‘Gala’ samples are plotted in the chart. Each data point is the average of two biological replicates and the bars indicate the standard deviation.Back to article page