Gene | Genomic size | Protein domain(s) | Amplicon size(s) | Primer name and sequence (5’-3’) | Length and portion of genomic sequence covered by EcoTILLING | Portion of reading frame covered by EcoTILLING |
---|---|---|---|---|---|---|
BvFL1 | 8.62 kb | MADS box/K box | FL1a: 977 bp FL1b: 632 bp | FL1a-fw tcggactttccctataagct FL1a-rv cacgtgaatcgttacagaca FL1b-fw gctgatagtctgtcccttttgtc FL1b-rv tgactccaacaccacgatgca | 1,609 bp (19%) | 62% |
BvFT1 | 7.53 kb | PEBP | FT1a: 916 bp FT1b: 713 bp | FT1a-fw tggtacgtgtatgaaacagaagctg FT1a-rv catcaactccatatttggggtg FT1b-fw acccatctatacttgtcgatgacc FT1b-rv caatggggaagtggttcacact | 1,629 bp (22%) | 92% |
BTC1 | 11.27 kb | REC, CCT | BTC1: 996 bp | BTC1-fw cagctgtaggatgttatcgtgctgag BTC1-rv agtaggtgataaggacaagacattgc | 992 bp (9%) | 15% |