Table 4 Interactors of AGP6 and AGP11 identified by Yeast two-Hybrid library screening assays

AGP6

At1g62750

−1.66

Elongation factor G (SCO1)

Apoplast [16]

At1g70810

−1.02

Calcium-dependent lipid-binding (CaLB domain) family protein

[5–7]

[5, 6]

At5g05010

−1.00

Clathrin adaptor complexes medium subunit family protein (MUG13.13)

[5–7]

[5, 6]

Plasmodesmata [17], cytosol [18], plasma membrane [19]

Predicted GPI-attachment [20], predicted secretory pathway [21], predicted beta-barrel TM domain [22]

At3g06650

−0.96

ATP-citrate lyase B-1

[7]

[6]

At1g32640

−0.95

MYC-related transcriptional activator (MYC2)

[5, 6]

No predicted binding site (cacatg), but two similar sites (acacaag) in AGP6 promoter region [23]

At1g67090

−0.93

Ribulose bisphosphate carboxylase small chain 1A (RBCS1A)

[5–7]

[5, 6]

Apoplast [16]

Predicted secretory pathway [21]

At5g55070

−0.91

Dihydrolipoamide succinyltransferase

[5], [6]

[5, 6]

Mitochondrion [24]

At2g20580

−0.86

26S proteasome regulatory subunit N1 (RPN1A)

[6]

[6]

Cytosol [18], plasmodesmata [17]

One to five predicted alpha-helix TM domains [22], ubiquitin-binding subunit of proteasome complex [25]

At3g61190

−0.85

BON1-associated protein 1 (BAP1)

[5], [7]

[5, 6]

Plasma membrane [26]

At3g09440

−0.83

Heat shock protein 70-3

[5–7]

[5, 6]

Cytosol [18], plasma membrane [27, 28], cell wall [29], tonoplast [30], Golgi [31], apoplast [16]

Predicted secretory pathway [21]

At5g21105

−0.77

L-ascorbate oxidase

[6]

[6]

Plasmodesmata [17], cell wall [32]

Predicted secretory pathway [21], predicted alpha-helix TM domains [22]

At5g28540

−0.52

Luminal-binding protein 1 (BIP1)

[5–7]

[5, 6]

Cell wall [29], cytosol [18], plasmodesmata [17], plasma membrane [27], ER [33], tonoplast [30], chloroplast [32]

Predicted secretory pathway [21], one to two predicted alpha-helix TM domains [22]

At5g57560

−0.43

Xyloglucan endotransglucosylase/hydrolase 22 (TCH4, XTH22)

[6]

Cell wall [24]

Predicted secretory pathway [21], predicted alpha-helix TM domain and predicted beta-barrel TM domain [22]

At5g15090

0.55

Mitochondrial outer membrane protein porin 2 (VDAC3)

[6]

[6]

Plasma membrane [19, 28], cell wall [29]

Predicted beta-barrel TM domain [22]

At1g16920

0.71

Ras-related small GTP-binding protein (RABA1b)

[7]

At3g18040

0.75

Mitogen-activated protein kinase 9 (MAPK9)

[5–7]

[5, 6]

Predicted GPI-attachment [20], predicted alpha-helix TM domain [22]

At4g05320

0.84

Polyubiquitin 10 (UBQ10)

[5–7]

[5, 6]

At2g20990

0.87

Synaptotagmin A (SYTA)

[7]

[6]

Endosome [34], plasma membrane [19, 28, 35], ER [32]

Predicted secretory pathway [21], predicted alpha-helix TM domain [22]

At1g26630

0.89

Eukaryotic translation initiation factor 5A-2 (FBR12)

[5–7]

[5, 6]

Predicted GPI-attachment [20]

At3g10740

0.92

Alpha-L-arabinofuranosidase 1 (ASD1)

[6], [7]

[6]

Apoplast [16], cell wall [36]

Predicted secretory pathway [21], one to two predicted alpha-helix TM domains [22]

At5g11200

1.22

DEAD-box ATP-dependent RNA helicase 56

[6]

[5, 6]

Cell wall [29], plasmodesmata [17]

At1g31150

2.50

Uncharacterized protein

[7]

Predicted secretory pathway [21], predicted alpha-helix TM domain [22]

AGP11

At3g47810

−1.43

MAG1 Homolog of yeast retromer subunit VPS29

[5–7]

[5, 6]

Multivesicular body [37], endosome [38]

Mediator of protein targeting to vacuole [39]

At1g42990

−0.97

bZIP transcription factor 60 (BZIP60)

[5–7]

[5, 6]

Predicted GPI-attachment [20], predicted alpha-helix TM domain [22]

At1g32640

−0.95

MYC-related transcriptional activator (MYC2)

[5, 6]

Two predicted binding sites in AGP11 promotor region [23]

At2g19070

−0.63

Spermidine hydroxycinnamoyl transferase (SHT)

Tapetum-specific [40], involved in exine formation [40, 41]

At2g30020

0.74

Putative protein phosphatase 2C 25

[5–7]

[5, 6]

Nucleus [42, 43], cytoplasm [43]