Table 5 Oligonucleotide primer sequences used in qPCR and in reverse-transcription PCR assays
AGI ID | primer ID | Forward primer | Reverse primer |
|---|---|---|---|
At4g05320 | UBQ10 | gctccgacaccattgacaac | acgcaggaccaagtgaagag |
At1g71380 | ATCEL3 | tctccaagtcattgctcttcttcc | cgttgtctcctgcgtcatagtac |
At3g60570 | ATEXPB5 | gcaaacggtgatgggaacttcg | ggacacggcggaggtaagc |
At5g11110 | ATSPS2F | tggtggtgttcgtgggagattcag | ttagcctcggtgatgttgggactg |
At1g53540 | HSP17.6C-CI | ggcaaacgcacccgctatg | ttcacctcttccttcctcagtcc |
AT2g46500 | AT2g46500 | agacggctcaaacgctcagaatc | cggatagggcttcgaggaatgc |
At2g39890 | PROT1 | atggcgagaggcgggtac | gtggttggctagaatgaatgtgag |
At1g50490 | UBC20 | agatcctccggcgtctaatgg | tgcttcctgttattgtccctttcc |
At5g60250 | AT5g60250 | cggttacagatggaggaggcactc | aggacaccacacgtagccacaatc |
At1g68610 | At1g68610 | ctctaacgaccaaccaagccaag | acatcgctccgctcacacc |
At4g27960 | UBC9 | gtttcaccaccctttcttc | aaatcccacgatcaaattcc |
At2g19970 | At2g19970 | gagacattctgatggaccttacg | tccgagacttaacgattgattgg |
At1g65760 | At1g65760 | cgatgattcctacattagcagac | aagacgcaacgggtaacg |
At2g22340 | At2g22340 | gcgacggtgggatttcaggaactg | gatgggagcggcaacggatgtg |
At5g28380 | At5g28380 | aagcacactgcgaccaaggc | cagcggctcatggatctcactac |
At4g09950 | At4g09950 | ggaggatgtgaaggagcaattagc | tcttgttgagttcggtgcgtaac |
At1g12840 | DET3 | cggcgttcttggcatgtgtc | agcaaggttgatagtgaaggagac |
At1g51490 | BGLU36 | gccgtactctctcgctgtcaaag | atgagccagaagttcgtgatgtcc |
At3g57880 | C2 domain-containing protein | caggatgaggtatrgacaggttgag | gcggcaatcaagcagaacaag |
At4g20780 | CML42 | cccaagcctaaacgcacttcg | acggtggatttgagatcggagag |
At2g19970 | CAP (Antigen 5) | gagacattctgatggaccttacg | tccgagacttaacgattgattgg |
At2g19980 | CAP (allergen V5) | gcacagaggtacgctaacg | ggtggcataattgtaataaggc |