AN knockout gene causes irregular seed pods, delay seed maturation and reduced silique number. (A-B) Seed pod pattern of wild type (A) and an-t1 (B) is represented. (C) At the same day after germination, the silique of wild type is maturing faster than that of an-t1. (D-E) At the same day after germination, the seeds of wild type are regularly arranged in the pod and maturing faster (D) than the seeds of an-t1 with irregular arrangement (having gaps: see arrows) and still green (E). (F), The total number of siliques per plant is higher in wild type than in an-t1. Asterisks indicate significant differences between mutant and wild type. *P <0.05, Student’s t test.