Organ-specific alterations in tobacco transcriptome caused by the PVX-derived P25 silencing suppressor transgene

BMC Plant Biology

Table 1 Microarray results verification by using quantitative real-time PCR (RT-qPCR)

Leaf (up regulated transcripts)		Microarray	RT-qPCR
EST/mRNA	Description	Log2	Log2	s.e.
EH617029	Capsicum annuum; WRKY transcription factor-30	5.87	5.71	0.15
EB438730	Dicer-like 2 splice form	2.09	2.17	0.16
EH620111	Pathogenesis-related protein 1B precursor	10.7	14.5	0.53
Flower (up regulated transcripts)
EH620111	Pathogenesis-related protein 1B precursor	4.0	5.71	0.51
Z11563	Acidic endochitinase precursor	2.53	2.75	0.41
Unchanged transcripts
EB450395	ARPC3 (actin-related protein C3)	−0.04
AM833694	DCL-1 (Dicer like 1)	0.003

Expression levels of some clearly up-regulated genes were tested by RT-qPCR. Statistical significance was tested using student t-test (p<0.05), with BH value of < 0.05. The depicted log2 values are normalized median intensive value (n=3) differences of wild type and P25-expressing transgenic plants. The Standard error of the mean (s.e.) is calculated for the C_t values of the RT-qPCR results.

ISSN: 1471-2229