Figure 5

Amino acid sites under neutral or positive selection in SOS1A. SOS1 proteins are generally under purifying selection but appear to have some residues under neutral or positive selection. However, these residues do not fall in the auto-inhibitory domain or in sites important for the activation of the protein. A – Amino acids under neutral or positive selection for each SOS1A ortholog, which are shown in the NJ tree on the left. B – A. thaliana SOS1A protein diagram based on Figure 2A and information obtained by others [47]. Amino acid residues predicted to be under neutral or positive selection are indicated by the broken lines. The grey area (residues 742–998) corresponds to the target-region of the auto-inhibitory domain, which is highlighted in dashed grey (residues 1013–1047). Amino acid residues 1136 and 1138 correspond to sites of recognition and phosphorylation, respectively, by the SOS2-SOS3 complex. TM: Trans-membrane region (residues 1–440). CP: Cytoplasmic region (residues 441–1146).