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Figure 4 | BMC Plant Biology

Figure 4

From: Progressive 35S promoter methylation increases rapidly during vegetative development in transgenic Nicotiana attenuata plants

Figure 4

Southern blot analysis of transgenic N. attenuata plants. Southern blot hybridization analysis was performed to detect the T-DNA copy number in the transgenic plant lines. Genomic DNA was isolated from homozygous seedlings and digested overnight in separate reactions with XbaI or EcoRV. A radiolabeled fragment of the hygromycin resistance gene (hptII) served as probe. The blot indicates the presence of two T-DNA insertions for lines PNA 6.7, PNA 1.2.1, PNA 10.1.1, ICE 4.4.1 and ICE 1.2. The fragment size from the DNA marker is indicated.

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