Cecropin A accumulates in dense subcellular fractions. A. Diagram of the sucrose (w/v) gradient steps. The total homogenate (T), supernatant (S) and the interfaces (1, 2, and 3) are indicated. B. Protein profiles of equivalent amounts of the recovered gradient fractions, separated in SDS gels and Coomassie-blue stained. C. Immunoblot analysis of equivalent amounts of gradient fractions isolated from untransformed (WT) and transgenic seeds carrying the genes indicated separated on tricine-SDS-gels and detected with anti-cecropin A antibodies.