Figure 4

qRT-PCR assays of the expression patterns of VvWRKY s under cold and exogenous ABA treatments. The default expression value for each gene was 1 at 0 hours before treatment. A, B and C represent the subgroups with different expression patterns in cold treatment and D represents the genes that up-regulated over 2 fold in ABA treatment. A: sustained up-regulated genes in cold treatment; B: genes that changes over 2 fold but without significant tendency in cold treatment; C: sustained down-regulated genes in cold treatment; D: up-regulated genes in exogenous ABA treatment. VvWRKYs that accumulated in both cold and exogenous ABA treatments were underlined. One-Way ANOVA analysis was used to test the impact of timing of cold treatment. When the effects were significantly different, we examined the difference between treatments using post hoc multiple comparisons (LSD, p < 0.05). All data analyses were conducted using IBM SPSS Statistics 20, and the results were displayed through a, b, c and d.